The practice of drinking the psychoactive drink ayahuasca has been shown in several studies to have positive long-term effects on mental states, and several studies have suggested it has a particularly strong positive effect on perceptions of identity. This research sought to discover if and in what way, these previous findings would be seen in gay people, who are often taught by their culture and religion that their lifestyles, values and sexual orientation are unacceptable. This qualitative study examined the interview responses of 17 gay and lesbian identified participants who had used ayahuasca in a group in the past three years regarding their self-perceptions. The results indicated that all participants reported positive effects on their lives from ayahuasca rituals, including affirmation of their sexual orientation, and no participants reported negative effects on perception of identity. KEYWORDS: Identity, Hallucinogenic drugs, Homosexuality, Religious practices