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Endogenous Hallucinogen Assay


Web link: www.cottonwoodresearch.org/crf-proje...

Abstract

An ultra-sensitive assay that will provide a deep view into the function of the naturally occurring tryptamine hallucinogens in humans, in both normal and non-normal states, is almost ready. We have established a simple method to, at present, separate, identify and quantify 26 compounds simultaneously in a single blood sample. Our current methodology provides us with the ability to prepare a blood sample for analysis as small as 100 microliters in as little as ten minutes and complete the sample analysis in as little as twenty minutes, all at the sub-nanogram level for the compounds being examined. The method is currently undergoing validation studies and we are continuing to examine ways to enhance overall assay sensitivity. The first application of this method will be in samples taken from consumers of ayahuasca, examining samples to confirm the metabolic pathways and to examine levels under enhanced conditions. This will be conducted in conjunction with the need to establish values in healthy men and women of all ages under “normal” physiological conditions. Circadian changes will also be examined. We will then begin the comparison of these values to those found in naturally occurring altered states of consciousness in which endogenous hallucinogens may play a role. These conditions and states include mania, schizophrenia, autism, depression, post-traumatic stress disorder, panic attacks, and dissociation. Such information may lead to important new breakthroughs in understanding and treating these disorders.