About 2000 species of Psychotria (Rubiaceae) can be found in the tropical and subtropical regions of the world. In Brazil, this genus is widespread and its species are found in all regions. Some of its species are important in herbal medicine, where they are used for infections of the female reproductive system, bronchitis, gastrointestinal disturbances, skin irritations, tumors, ulcers, and eye disturbances. Psychotria barbiflora DC., a synonym of Psychotria hoffmannseggiana (Willd. ex Aschult.) Müll. Arg., is a small shrub with height varying from 0.5 to 2.0 m, found from the South to the Northeast Brazilian. Leaves and stems of this plant were collected from Parque Municipal de Maceió-AL in May 2008 and identified by botanist Rosangela P. Lyra Lemos, at the Instituto do Meio Ambiente do Estado de Alagoas (IMA-AL), Maceió-AL, Brazil, where a voucher specimen (MAC-11353) is deposited. Harmane and Stricnosidic acid were identified iin the leaves.