The present article reports the accumulation of N,N-dimethyltryptamine and its metabolic precursors (tryptophan, tryptamine) in different organs of micropropagated Mimosa tenuiflora trees (leaves, flowers and bark) subjected to seasonal variations (January and June), as well as in in-vitro cultures (plantlets and calluses) of this plant species. The accumulation of all the tested compounds varied according to the organ, the month of collection, and age of the plant material. In all cases, the neurotoxic compound N,N-dimethyltryptamine (DMT) was detected with the lowest concentration 0.01% dry weight (DW) in flowers, and the highest 0.33% DW in bark. For the in-vitro cultures, DMT was present in high yields in plantlets (0.1–0.2% DW), while in calluses this compound was initially detected but its concentration decreased significantly in the sub- sequent subcultures. Keywords: In-vitro cultures; Mimosa tenuiflora; N,N-dimethyltryptamine (DMT); Tepescohuite; Tryptamine derivatives