Dimethyltryptamine (DMT) was extracted from the root bark of Mimosa hostilis via three methods, using methanol (direct or via Soxhlet) and acetic acid (direct only), respectively. The product from the direct methanol extraction was used in both qualitative and quantitative analysis, while the product from the acetic acid extraction (isolated in crystal form after workup) was used for qualitative analysis. FTIR/ATR, GC/MS, GC/IRD, 1H-NMR, and HPLC data are presented. Quantitative analysis by 1H-NMR and HPLC indicated 0.9 percent and 0.8 percent DMT, respectively, in the analyzed samples.