This paper focuses on glocal trends in ayahuasca utilization with reference to the Czech Republic. Specifically, current tendencies in glocalization of this traditional Amazonian medicine are described. Field work was carried out in the Czech Republic from November 1, 2015 to December 4, 2016. The paper contains classification of local rituals, characteristics of the organizers and their working styles. The research sample consists of 46 persons (23 women and 23 men) who at least once took part in some type of the ayahuasca ritual. Semi-structured interviews with an average length of 50 minutes were recorded with informants. These interviews were subsequently analyzed using the Grounded theory method. The results confirm that rituals are adapted according to the needs of the ayahuasca users. These users participate in the rituals not only because of healing, but also for curiosity/experiment, personality development and spiritual and religious reasons. Finally, some risks related to ayahuasca utilization in local conditions are discussed.