Sensitive and reproducible modifications of paper and thin layer chromatographic methods have been developed for detecting N -methylated indoleamines in the urine. The methods consist of three main steps: (I) a preliminary concentration of urine, followed by (2) the purification of indoleamine fractions using an ion-exchange resin, and (3) identification procedures, By the present methods, free and conjugated indoleamines could be examined separately, and as little as I I_cgof indoleamines per 1000 ml urine could be consistently detected. The A$ values and the color of several indoleamines and related compounds on paper and thin layer chromatograms, are presented. The usefulness of these methods was proved in the analysis of schizophrenic urines, in which bufotenin, N-methylserotonin, N-dimethyltryptamine and 5methoxy N-dimethyltryptamine were revealed when the patients were receiving a monoamine oxidase inhibitor.